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SIH Amino acid drop-out mixture is based on the Amino acids present in SIH Minimal medium. These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants. Each SIH Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the "dropped out" supplements. SIH Amino acids drop-out mixtures are used in combination with SIH Minimal medium w/o Amino acids or SIH Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium.
Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes.
Formula |
mg/l
|
| Amino Acids | |
| Arg | 700 |
| Asp | 300 |
| Asp A | 150 |
| Cys | 300 |
| GluA | 545 |
| Gly | 900 |
| His | 300 |
| Ile | 600 |
| Leu | 900 |
| Lys | 1250 |
| Met | 350 |
| Phe | 550 |
| Pro | 800 |
| Thr | 500 |
| Trp | 350 |
| Val | 700 |
| Total | 9195 mg/l |
Please enquire about custom made formulations.
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