Iscove’s Modified Dulbecco’s Medium is based on DMEM High Glucose, containing selenium, HEPES, and, instead of iron, potassium (as potassium nitrate) besides additional amino acids and vitamins. It is well suited for culturing of a wide range of mammalian cell lines and primary cells, including B- and T lymphocytes as well as hybridoma cells.

When working without FBS, growth factors, hormones and lipids, which are essential for cell growth, must also be added to the medium.

This modification of IMDM contains L-glutamine, pyruvate and 25 mM HEPES.