Versatile marker for depiction of all standard DNA fragment lengths.Manufactured by restricting unmethylated DNA with the restriction endonucleases Hind III and Hae III.By mixing high molecular weight Lambda (Hind III) fragments with short-sequence phiX-174 (Hae III) fragments a marker was designed which covers a broad range from 200 bp up to 20 kb. Most fragments are distributed in an equimolar way, additionally allowing rough DNA quantification in the gel. Due to this equimolarity, however, the smallest bands of 125 bp, 118 bp and 72 bp can not be detected efficiently in standard gels.

Optimal results are obtained with a running distance of approx. 10 cm.

Fig.: 1 % agarose (NEEO, Art. No. 2267).