Ligase T4, 5 U/µl, for Molecular Biology, 100 µl, plasticLigase T4, 5 U l, for Molecular Biology, Density (D) 1,18 g cm, Storage temp. 20 C ATP dependent recombinant ligase for molecular cloning, site directed mutagenesis, pitch repair in duplex DNA, RNA or DNA RNA hybrids and ligation mediated PCR. Cloning of PCR and restriction fragments Self circulation of linear DNA Site directed mutagenesis T4 DNA Ligase catalyses the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl end