Kit for amplification of immunochemical detection signals on blot membranes and in ELISA.

The Signal Enhancer Western/ELISA enhances antigen-antibody reactions in a variety of immunoassays such as Western blotting, dot blotting and ELISA. It can significantly enhance detection of weak immunoreactive and low abundance target proteins and is well compatible with all common assay versions, membranes and ELISA plates. Signal enhancement is dependent on several parameters such as the particular antigen/antibody pair, the particular detection enzyme, the substrate chosen and so on. In most cases, however, the signal strength is about 5 to 10fold higher than with the substrate alone.

For application, the two solutions Signal Enhancer Western/ELISA-1 and -2 are simply used as buffer solutions for preparation of the primary- and secondary antibody incubation solutions. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI^®DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI^®Lumin plus (Art. No. 3692).

The solution Signal Enhancer Western/ELISA-2 enhances binding of secondary antibodies in a variety of immunoassays such as Western blotting, dot blotting and ELISA. Even for weak immunoreactive and low abundance target proteins strong signals may thus be achieved. The solution is well compatible with all common assay versions, membranes and ELISA plates.Recommended for performance of assays using one antibody (labelled) only.

For application, the solution Signal Enhancer Western/ELISA-2 is simply used as buffer solutions for preparation of the secondary antibody incubation solution. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI^®DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI^®Lumin plus.