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home > anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic > anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic

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anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plasticanti DNA mouse monoclonal,, AC 30 10, 100 g ml, affinity purified (made by PROGEN), Density (D) ~1 g cm, Boiling point (bp) ~100 C, Storage temp. +4 C This antibody portfolio includes a wide range of tissue & tumor specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of

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anti-DNA mouse monoclonal,, AC-30-10, 100 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures. Binding characteristics: reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.

Application

Dot Blot: Assay dependent (on nitrocellulose membrane, after baking at 70°C) Immunocytochemistry (ICC): 1:200 (0.5 μg/ml) Immunohistochemistry (IHC) - frozen: 1:10 (10 μg/ml) Immunohistochemistry (IHC) - paraffin: 1:10 (10 μg/ml, microwave treatment recommended)

Immunofluorescence analysis of HeLa cells with anti-DNA antibody. Fixation was performed using 3% paraformaldehyde for 10 min at RT. Cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT and permeabilized with 0.3% Triton-X 100 in PBS for 10 min at RT. The primary antibody anti-DNA mouse monoclonal, AC-30-10 (Cat. No. 690014) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated over-night at 4°C. The secondary antibody goat anti-mouse IgM Alexa488 was also diluted in blocking buffer (antibody concentration 2.5 μg/ml) and incubated for 30 min at 37°C and 30 min at RT. Nuclear DNA was stained with DAPI in blue.

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC Dot Blot
Host species	mouse
Clonality	monoclonal
Reactivity antibody	species-independent
Purification	Size-exclusion chromatography
Conjugate	uncojugated
Concentration	100 µg/ml
Isotype	IgM
Clone	AC-30-10
Target	double- and single-stranded DNA

Technical Documentation (PDF)

anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic

Name: anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic
Brand: www.midwoodautocraft.com
SKU: 44530825693
Price: 130.65 USD
Availability: InStock
Rating: 4.4446 (2225 reviews)