Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.

The ROTI^®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI^®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.

ROTI^®GelStain is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels.

The dye can be used in a similar manner as ethidium bromide. By adding the dye to the liquid gel, the nucleic acids are stained during the gel run.ROTI^®GelStain has a sensitivity of 0.2 ng/band and is thus even slightly more sensitive than ethidium bromide.When bound to nucleic acids in agarose gels, ROTI^®GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR^® Green foto filters.ROTI^®-GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection.

Excitation maximum (bound to DNA): 302 nm (UV) and 490 nm (blue light)Emission maximum (bound to DNA): 520 nm