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home > anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic > anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic

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anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plasticanti Cyclin Dependent Kinase 4, mouse monoclonal, DCS 156, 50 g ml, affinity purified (made by PROGEN), Density (D) ~1 g cm, Boiling point (bp) ~100 C, Storage temp. +4 C This antibody portfolio includes a wide range of tissue & tumor specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the

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anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

DCS-156 reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. In immunohistochemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) and cervix DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation. Epitope recognized: aa 270-290.

Application

Western Blot (WB): 1:50-1:500 (0.1-1 μg/ml)

Western blot analysis of human HeLa cell lysate with anti-Cyclin-Dependent Kinase 4 antibody. Western blot analysis was performed on either 5 μg or 10 μg of HeLa lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Cyclin-Dependent Kinase 4 mouse monoclonal, DCS-156 (Cat. No. 690096) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Not a medical device / Not an IVD product

Technical Information

Application	WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	mouse human rat
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG1
Uniprot ID AK	P11802 (human),P30285 (mouse),P35426 (rat)
Clone	DCS-156
Target	human recombinant full-length cdk4 polypeptide

Technical Documentation (PDF)

anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic

Name: anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic
Brand: www.midwoodautocraft.com
SKU: 51243144964
Price: 109.15 USD
Availability: InStock
Rating: 4.8748000000000005 (979 reviews)