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home > 3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass > 3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass

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3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass3,3',5,5' Tetramethylbenzidine, min. 98 %, p. a., Molar mass (M) 240,35 g mol, Storage temp. +4 C, CAS No. 54827 17 7, EC No. 259 364 6, Empirical formula C16H20N2 Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine. Dissolves easily in acetone, chloroform and ethanol. Directions for use Working solution: 1 mg TMB in 0. 1 ml dimethyl sulfoxide, add 9. 9 ml 0. 1 M sodium acetate (pH

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3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., Molar mass (M) 240,35 g/mol, Storage temp. +4 °C, CAS No. 54827-17-7, EC No. 259-364-6, Empirical formula C₁₆H₂₀N₂

Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.

Dissolves easily in acetone, chloroform and ethanol.

Directions for use

Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H₂O₂ ad 0.01 %. Always prepare freshly!Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H₂SO₄ and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187).Protect from light.

Technical Information

Enzyme	Horseradish Peroxidase
Detection	Colour (blue)
Signal product	soluble (ELISA)

Technical Documentation (PDF)

3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass

Name: 3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass
Brand: www.midwoodautocraft.com
SKU: 73141521809
Price: 70.45 USD
Availability: InStock
Rating: 4.3195 (2349 reviews)