The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.

ROTI^®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.

ROTI^®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI^®Load DNA. ROTI^®Load DNA gel loading buffer is usually used as 1x concentrated solution.

ROTI^®Load DNA-orange 1 (with glycerol) is a special gel loading buffer with orange G as the only dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs (see lane 6 in the product image). Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands.

Glycerin is the standard reagent for increasing sample density.

Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.