ROTI^®Prep Soil DNA was developed to isolate microbial DNA from soil samples.Soil samples represent a particularly complex starting material, which is why it is urgently necessary to first mechanically decompose it. In the first step of extraction, the soil samples are homogenised and disrupted in a lysis tube in lysis buffer. After a centrifugation step, the lysate is transferred to the centrifugation column and the DNA is bound to the membrane with the aid of the binding buffer. After several washing and centrifugation steps, the DNA is then eluted from the membrane with the elution buffer.

ROTI^®SampleLyse Microbes 1 tubes, Lysis Buffer LSS, Precipitation Buffer PB, Binding Buffer BR, Binding Buffer BDS, Washing Solution WSO, Washing Buffer WSH (conc.), Elution Buffer EB, detailed instructions.Ethanol 99.8 % (p.a., Art. no. 9065) and a homogeniser (e.g. Art. no. PA66.1) are not included in the kit and should be ordered separately if required.