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Safranin O (C.I. 50240), for microscopy, Molar mass (M) 350,85 g/mol, CAS No. 477-73-6, EC No. 207-518-8, Empirical formula C20H19ClN4
Basic azin dye, soluble in water and ethanol, well adapted to visualise chromosomes. Safranin is used for bacteria staining, e.g. during Gram-staining or spore staining acc. to Wirtz.
| Ox. colour | blue-violet (acid) brown (alkaline) |
| Red. colour | colourless |
| Redox potential E0[V] at pH=0 and 20 °C | +0.24 |
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