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home > ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, 1 kit > ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, 1 kit
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ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, 1 kitROTIPHORESE DNA sequencing system, for 50 sequencing gels 30 x 40 cm, for DNA sequencing, Density (D) 1 g cm, Storage temp. +4 to +15 C For preparation of 4 20 % sequencing gels Most simple handling of three optimised ready to use solutions No tedious dissolving of urea Prevents acrylamide dusts Reproducible, superior quality of gels In tried and tested ROTIPHORESE quality Convenient set containing three ready made solutions for easy and fast casting
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ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, for DNA sequencing, Density (D) 1 g/cm³, Storage temp. +4 to +15 °C

  • For preparation of 4-20 % sequencing gels
  • Most simple handling of three optimised ready-to-use solutions
  • No tedious dissolving of urea
  • Prevents acrylamide dusts
  • Reproducible, superior quality of gels
  • In tried-and-tested ROTIPHORESE® quality

Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®Sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %.

Directions for use

Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).

The kit contains:
  • 1 l ROTIPHORESE®sequencing gel concentrate (Art. No. 3043.1) - ready-to-use, stabilised solution of 25 % acrylamide/bisacrylamide 19:1 with 50 % urea (8,3 M).
  • 1 l ROTIPHORESE®sequencing gel diluent (Art. No. 3047.1) - 50 % (g/v; 8,3 M) solution of urea in water.
  • 250 ml ROTIPHORESE®sequencing gel buffer concentrate (Art. No. 3050.1) - 10x TBE with 50 % urea (8,3 M).
  • Detailed instructions for use are enclosed with the product.

ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, 1 kit

Item no : 98091357993
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US$ 142.45
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