PerCP/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1]

Size: 100μg

Catalogue Number: E-AB-F1112UJ-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD45R/B220

Target Synonym: B220,

Conjugation: PerCP/Cyanine5.5

Host: Rat

Species Reactivity: Mouse

Application: FCM

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10^6 cells in 100 μL volume].

Isotype: Rat IgM, κ

Clone No.: RA3.3A 1/6.1

UNIProt ID: ???

Background: CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity.

Concentration: 0.2 mg/mL

Control Catalogue Number: E-AB-F09773J

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

GeneID: 192, 645, 788

Storage: This product can be stored at 2-8°C for 12 months. Please protect from prolonged exposure to light and do not freeze.

Shipping: Ice bag