QuicKey Human CLU (Clusterin) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-TSEL-H0014-24, E-TSEL-H0014-48, E-TSEL-H0014-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: CLU

UNIProt ID: P10909

Target Synonyms: Apo-J; APOJ; KUB1; CLI; NA1/NA2; SGP-2; TRPM-2; Aging-associated gene 4 protein; Apolipoprotein J; Complement cytolysis inhibitor; Complement-associated protein SP-40; 40; Ku70-binding protein 1; Sulfated glycoprotein 2; Testosterone-repressed prostate message 2;

Research Areas: Immunology,Cardiovascular,Metabolism,Signaling transduction

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human CLU concentrations in Serum, Plasma, Urine, Saliva.

Tested Sample Types: Serum, Plasma, Urine, Saliva

Type: Sandwich

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 0.94 ng/mL

Detection Range: 1.56-100 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CLU. You can calculate the concentration of Human CLU in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human CLU in samples. No significant cross-reactivity or interference between Human CLU and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃, 6 months

Shipping: ice bag