ADAM19 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS60747-50, BS60747-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9H013

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: The ADAM (a disintegrin and metalloprotease) protein family, which includes over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases, plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFα (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. The ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in a wide range of mammalian tissues and several are abundantly expressed in the male reproductive tract . Expression of ADAM19, also designated meltrin b, is highest in the peripheral nervous system during embryogenesis, but is also apparent in placenta, brain, heart, lung, leukocytes, and SW480 cells . ADAM19 also serves as a dendritic cell marker. Truncation of ADAM19 in its cysteine-rich domain is necessary to exert its proteolytic activity on specific substrates, including a2-macroglobulin .

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ADAM19 polyclonal antibody detects endogenous levels of ADAM19 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ADAM19 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate, Immunohistochemistry (IHC) analyzes of ADAM19 pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 105 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Disintegrin and metalloproteinase domain-containing protein 19; ADAM 19; Meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MADDAM; ADAM19; MLTNB; FKSG34

Immunogen: A synthetic peptide corresponding to residues in Human ADAM19

Conjugate: Unconjugated

Modification: Unmodified