Human HSP90b1 (Heat Shock Protein 90kDa Beta 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1609-48, ELK1609-96, ELK1609-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen (gp96)1; 94 kDa glucose-regulated protein; Endoplasmin

Assay Type: Sandwich

Sensitivity: 0.133 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Immune molecule; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP90b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP90b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP90b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP90b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P14625

Research Use Only