CD50/ICAM-3 Recombinant Protein

Catalogue Number: NCP0215-500, NCP0215-1000

Sizes: 500ug, 1mg

Swiss-Prot: P32942

Host: E.coli

Tag: His-tag

AA Sequence: CAGGAATTCCTGCTGCGCGTGGAACCGCAGAATCCGGTTCTGAGTGCCGGCGGTAGTCTGTTCGTTAATTGCAGTACCGATTGTCCGAGCAGTGAAAAAATTGCACTGGAAACCAGCCTGAGCAAAGAACTGGTTGCCAGCGGCATGGGTTGGGCAGCATTCAATCTGAGCAATGTGACCGGCAATAGTCGTATTCTGTGTAGCGTGTATTGTAATGGTAGTCAGATTACCGGCAGTAGCAATATTACCGTGTATCGTCTGCCGGAACGCGTGGAACTGGCCCCGCTGCCTCCGTGGCAGCCTGTTGGTCAGAACTTCACCCTGCGTTGCCAGGTGGAAGATGGCAGCCCGCGTACCAGTCTGACCGTGGTGCTGCTGCGCTGGGAAGAAGAACTGAGTCGTCAGCCGGCAGTTGAAGAACCGGCAGAAGTGACCGCCACCGTGCTGGCAAGTCGTGATGATCATGGCGCCCCGTTCAGTTGCCGTACCGAACTGGATATGCAGCCGCAGGGTCTGGGTCTGTTCGTTAACACCAGTGCACCGCGTCAGCTGCGTACCTTCGTTCTGCCGGTGACCCCGCCGCGCCTGGTTGCACCTCGCTTCCTGGAAGTTGAAACCAGTTGGCCGGTGGATTGCACCCTGGATGGCCTGTTCCCGGCCAGTGAAGCCCAGGTGTATCTGGCACTGGGTGATCAGATGCTGAATGCCACCGTTATGAATCATGGCGATACCCTGACCGCCACCGCAACCGCAACCGCGCGTGCAGATCAGGAAGGTGCACGCGAAATTGTGTGTAATGTGACCCTGGGCGGCGAACGTCGCGAAGCACGCGAAAATCTGACCGTGTTCAGCTTCCTGGGTCCGATTGTGAATCTGAGCGAACCGACCGCACATGAAGGCAGCACCGTGACCGTGAGTTGCATGGCAGGCGCACGCGTGCAGGTGACCCTGGATGGTGTGCCGGCCGCCGCACCTGGTCAGCCTGCACAACTGCAGCTGAATGCAACCGAAAGCGATGATGGTCGTAGCTTCTTCTGCAGCGCAACCCTGGAAGTTGATGGCGAATTCCTGCATCGTAATAGTAGCGTTCAGCTGCGTGTGCTGTATGGTCCGAAAATTGATCGTGCAACCTGTCCGCAGCATCTGAAATGGAAAGATAAAACCAGACATGTTCTGCAGTGTCAGGCACGTGGTAATCCGTATCCGGAACTGCGTTGTCTGAAAGAAGGTAGTAGTCGCGAAGTTCCGGTTGGTATTCCGTTCTTCGTTAATGTGACCCATAATGGCACCTATCAGTGTCAGGCCAGCAGTAGCCGCGGCAAATATACCCTGGTGGTGGTGATGGATATTGAAGCAGGCAGCAGTCAT

Restriction Sites: NdeI-XhoI

Background: Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth. These proteins are thought to play an important role in embryogenesis and development. ICAM-3, also designated CD50 and ICAM-R, is a type I membrane protein that is thought to regulate morphological changes during cell locomotion. ICAM-3 acts as a counter-receptor for the leukocyte Integrin αL/β2, and is known to activate T cells and polymorphonuclear leukocytes. ICAM-3 also binds to Moesin, via the cytoplasmic domain of ICAM-3. The expression of ICAM-3 is induced by RANTES, a chemoattractant known to activate T lymphocytes. ICAM-3 is also a major ligand for the leukocyte Integrin LFA-1 (CD11a/CD18).

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~50kDa

Notes: For research use only, not for use in diagnostic procedure.