SARS-CoV-2 Spike S2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77972-50, BS77972-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P0DTC2

Host: Rabbit

Reactivity: SARS-CoV-2

Applications: WB, IP

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:200

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It''s been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 36KDa

Immunogen: A synthetic peptide of SARS-CoV-2 Spike S2(YP_009724390.1).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike S2 Protein, using SARS-CoV-2 Spike S2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S2 antibody. Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 antibody at a dilution of 1:3000.