CAS9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79938-50, BS79938-100

Host: Rabbit

Reactivity: Human, Other (Wide Range)

Applications: WB, IF/ICC

All Applications: WB,1:5000 - 1:10000 | IF/ICC,1:50 - 1:200

Background: Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of 293T cells and 293T transfected with CAS9 protein, using CAS9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s., Immunofluorescence analysis of HeLa cells transfected with Cas9 and untreated HeLa cells using CAS9 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Bioworld Molecular Weight: 160KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CAS9

Immunogen: Recombinant fusion protein of streptococcus CAS9(NP_269215.1).

Conjugate: Unconjugated

Modification: Unmodified