TPH1 (Phospho-S58) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64532-50, BS64532-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17752

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TPH1 (Phospho-S58) polyclonal antibody detects endogenous levels of TPH1 protein only when phosphorylated at Ser58.

Bioworld Molecular Weight: ~ 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TPH1 (Phospho-S58) polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate
Lane B: THP-1 whole cell lysate
Lane C: MCF-7 whole cell lysate
Lane D: SGC7901 whole cell lysate

Alternative Name: Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH

Immunogen: Synthetic phosphopeptide derived from human TPH1 around the phosphorylation site of Serine 58.

Conjugate: Unconjugated

Modification: Phosphorylation