Tth DNA Ligase catalyzes the NAD-dependent formation of phosphodiester bonds between adjacent 3'-hydroxyl and 5'-phosphate termini in double-stranded DNA. This ligase is inactive against single-stranded DNA or RNA and blunting DNA.

The Tth DNA ligase is stable and active at much higher temperatures than conventional DNA ligases. The optimal ligation temperature range is 7-10 °C higher than that of the T4 DNA ligase and is determined by the Tm of the substrates. High ligation temperature eliminates the nonspecific ligation.

Quick LigaseLigation buffer (10x)The individual components of this kit cannot be purchased separately.The tubes included in the kit are colour coded to avoid confusion of reagents.